• Species Reactivity
  Mouse
• Specificity
  Detects mouse MR1 in direct ELISAs.
• Source
  Monoclonal Rat IgG_2A Clone # 909417
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant mouse MR1
  Met1-Arg296
  Accession # Q8HWB0
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Immunohistochemistry
  8-25 µg/mL
  See below

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Major Histocompatibility Complex, Class I-Related
• Entrez Gene IDs:
  3140 (Human); 15064 (Mouse)
• Alternate Names:
  Class I histocompatibility antigen-like protein; FLJ31593; H2ls; HLALS; major histocompatibility complex class I-related gene protein; major histocompatibility complex, class I-like sequence; major histocompatibility complex, class I-related; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; MR1

Background:

The Major Histocompatibility Complex (MHC) class I-related gene, MR1, considered a non-classical MHC class IA gene which is encoded outside the MHC region. The MR1 is responsible for activation of Mucosal-associated Invariant T (MAIT) cells expressing semi-invariant T cell receptors in the presence of bacteria. MR1 is a highly evolutionary conserved protein with regard toalpha 1 andalpha 2 domains corresponding to the peptide-binding domains of classical MHC class I molecules which show about 90% amino acid identity between human and mouse. MR1 protein can associate withbeta 2-microglobulin, which indicates that this is a typical class I heterodimer composed of a heavy and a light chain resembling the classical MHC class I molecules.